Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma.

@article{Lindegrdh2005DevelopmentAV,
  title={Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma.},
  author={Niklas Lindeg{\aa}rdh and Anna Annerberg and Daniel Blessborn and Yngve Bergqvist and Nicholas P J Day and Nicholas J White},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2005},
  volume={37 5},
  pages={1081-8}
}
A bioanalytical method for the determination of lumefantrine (LF) and its metabolite desbutyl-lumefantrine (DLF) in plasma by solid-phase extraction (SPE) and liquid chromatography has been developed. Plasma proteins were precipitated with acetonitrile:acetic acid (99:1, v/v) containing a DLF analogue internal standard before being loaded onto a octylsilica (3 M Empore) SPE column. Two different DLF analogues were evaluated as internal standards. The compounds were analysed by liquid… CONTINUE READING

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