Development and validation of a Myxoma virus real-time polymerase chain reaction assay

@article{Albini2012DevelopmentAV,
  title={Development and validation of a Myxoma virus real-time polymerase chain reaction assay},
  author={Sarah Albini and Brigitte Sigrist and Regula G{\"u}ttinger and C Schelling and Richard K. Hoop and Andrea V{\"o}gtlin},
  journal={Journal of Veterinary Diagnostic Investigation},
  year={2012},
  volume={24},
  pages={135 - 137}
}
To aid in the rapid diagnosis of myxomatosis in rabbits, a real-time polymerase chain reaction (PCR) for the specific detection of Myxoma virus is described. Primers and probe were designed to amplify a 147-bp fragment within the Serp2 gene. The assay was able to detect 23 copies of a synthesized oligo indicating a reliable sensitivity. In addition, the real-time PCR did not detect the Rabbit fibroma virus used in myxomatosis vaccines. The novel PCR was shown to be able to detect Myxoma virus… 
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