Development and validation of SSR markers for Coffea arabica

  title={Development and validation of SSR markers for Coffea arabica},
  author={Robson Fernando Missio and Eveline Teixeira Caixeta and Eunize Maciel Zambolim and La{\'e}rcio Zambolim and Ney Sussumu Sakiyama},
With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four… CONTINUE READING

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