Development and validation of GC–MS methods for the comprehensive analysis of amino acids in plasma and urine and applications to the HELLP syndrome and pediatric kidney transplantation: evidence of altered methylation, transamidination, and arginase activity

@article{Hanff2018DevelopmentAV,
  title={Development and validation of GC–MS methods for the comprehensive analysis of amino acids in plasma and urine and applications to the HELLP syndrome and pediatric kidney transplantation: evidence of altered methylation, transamidination, and arginase activity},
  author={Erik Hanff and Stephan Ruben and Martin Kreuzer and Alexander Bollenbach and Arslan Arinc Kayacelebi and Anibh Martin Das and Frauke von Versen-H{\"o}ynck and Constantin S. Kaisenberg and Dieter Haffner and Stefan {\"U}ckert and Dimitrios Tsikas},
  journal={Amino Acids},
  year={2018},
  volume={51},
  pages={529-547}
}
We developed and validated gas chromatography–mass spectrometry (GC–MS) methods for the simultaneous measurement of amino acids and their metabolites in 10-µL aliquots of human plasma and urine. De novo synthesized trideutero-methyl esters were used as internal standards. Plasma proteins were precipitated by acidified methanol and removed by centrifugation. Supernatants and native urine were evaporated to dryness. Amino acids were first esterified using 2 M HCl in methanol and then amidated… Expand
20 Citations
Development, validation of a GC-MS method for the simultaneous measurement of amino acids, their PTM metabolites and AGEs in human urine, and application to the bi-ethnic ASOS study with special emphasis to lysine.
TLDR
The GC-MS method was developed and validated in relevant concentration ranges and used to measure the urinary excretion rates of Lys and its PTM metabolites and AGEs in healthy black and white boys of the Arterial Stiffness in Offspring Study (ASOS). Expand
GC-MS and LC-MS/MS pilot studies on the guanidine (NG)-dimethylation in native, asymmetrically and symmetrically NG-dimethylated arginine-vasopressin peptides and proteins in human red blood cells.
TLDR
The LC-MS/MS proteomic study identified several membrane and cytosolic erythrocytic NG-dimethylated proteins, including spectrin-α (280 kDa),Spectrin-β (247 k da) and protein 4.1 (80 k Da) as newly identified main targets for NG-Dimethylation in human ery Throcytes should be given a closer look in ery throatcytic diseases like hereditary spherocytosis. Expand
Measurement of the tripeptides glutathione and ophthalmic acid by gas chromatography-mass spectrometry.
TLDR
The GC-MS method demonstrated that in human hemolysate, nitrite at toxicological concentrations (range, 0 - 15 mM) depletes erythrocytic GSH and suggesting lack of interference by GSSG. Expand
Urinary excretion of amino acids and their advanced glycation end-products (AGEs) in adult kidney transplant recipients with emphasis on lysine: furosine excretion is associated with cardiovascular and all-cause mortality
TLDR
It is indicated that lower urinary CML and lower furosine excretion rates are associated with higher mortality in KTR, thus resembling the associations of ADMA. Expand
Evidence by GC-MS that lysine is an arginase-catalyzed metabolite of homoarginine in vitro and in vivo in humans.
TLDR
Results suggest that Lys is a metabolite of hArg produced by the hydrolytic activity of arginase, which may play a key role in hArg homeostasis in humans. Expand
Profile of urinary amino acids and their post-translational modifications (PTM) including advanced glycation end-products (AGEs) of lysine, arginine and cysteine in lean and obese ZSF1 rats.
TLDR
It is suggested that hyperglycemia, hyperlipidemia and elevated oxidative stress in the O-ZSF1 rats favor PTM methylation of arginine and lysine and the glycation of lysines and cysteine. Expand
GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO2-NH2) drugs as pentafluorobenzyl derivatives [R-SO2-N(PFB)2] and quantification of pharmacological acetazolamide in human urine
TLDR
It is demonstrated for the first time that sulfonamide drugs can be derivatized with PFB bromide and quantitated by GC-MS and methazolamide was also found to form a N,N-dipentafluorobenzyl derivative, whereas dorzolamide (MM, 324) was hardly detectable. Expand
GC-MS Discrimination of Citrulline from Ornithine and Homocitrulline from Lysine by Chemical Derivatization: Evidence of Formation of N5-Carboxy-ornithine and N6-Carboxy-lysine
TLDR
It is demonstrated that the derivatization procedure B enables discrimination between Cit and Orn, and between hCit and Lys, and the utility of procedure B to measure simultaneously these amino acids in biological samples such as plasma and urine remains to be demonstrated. Expand
Diminished Systemic Amino Acids Metabolome and Lipid Peroxidation in Ureteropelvic Junction Obstruction (UPJO) Infants Requiring Surgery
TLDR
A diminished amino acids metabolome is indicated in the serum of UPJO infants requiring surgery comparing to a conservative group, and the concentrations of the majority of the biomarkers were found to be lower in Group A compared to Group B. Expand
GC–MS measurement of spermidine and putrescine in serum of elderly subjects: intriguing association between spermidine and homoarginine
TLDR
The results suggest that SPD and PUT synthesis is associated with hArg formation and protein citrullination in healthy elderly subjects and the mechanisms underlying these associations remain to be elucidated. Expand
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