Development and utility of PCR-based intron polymorphism markers in sorghum [Sorghum bicolor (L.) Moench]

@article{Jaikishan2015DevelopmentAU,
  title={Development and utility of PCR-based intron polymorphism markers in sorghum [Sorghum bicolor (L.) Moench]},
  author={Inapakurti Jaikishan and Passoupathy Rajendrakumar and R Madhusudhana and Maruthamuthu Elangovan and Jagannath Vishnu Patil},
  journal={Journal of Crop Science and Biotechnology},
  year={2015},
  volume={18},
  pages={309-318}
}
Sequence variations involving DNA insertions and deletions within introns provide suitable targets for the development of PCR-based markers. In this study, 37861 potential introns were identified in sorghum and primers were designed to develop intron length polymorphism (ILP) markers. Drastic variation was observed in the number of ILP markers on each chromosome with a range of 1498 (chr. 5) - 7290 (chr. 1), which was also reflected in the fluctuations in their density. About 200 ILP markers… CONTINUE READING