Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain.

@article{FernndezGarca2010DevelopmentAU,
  title={Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain.},
  author={Aurora Fern{\'a}ndez-Garc{\'i}a and Gema {\'A}lvarez-Garc{\'i}a and Ver{\'o}nica Risco-Castillo and Adriana Aguado-Mart{\'i}nez and Jos{\'e} Miguel Marc{\'e}n and Silvia Rojo-Montejo and Juan Antonio Castillo and Lu{\'i}s Miguel Ortega-Mora},
  journal={The Veterinary record},
  year={2010},
  volume={166 26},
  pages={818-22}
}
An indirect ELISA based on a soluble extract of Besnoitia besnoiti tachyzoites was developed and standardised. A set of positive and negative reference bovine sera were characterised using an immunofluorescence antibody test and Western blot. A cut-off with a relative index per cent of 8.1 was determined for equal sensitivity and specificity (100 per cent) by two-graph receiver operating characteristic analysis. Cross-reactions with other closely related Apicomplexan parasites were discarded… CONTINUE READING

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