Development and testing of improved suicide functions for biological containment of bacteria.

@article{Knudsen1995DevelopmentAT,
  title={Development and testing of improved suicide functions for biological containment of bacteria.},
  author={Steen Knudsen and Peter Saadbye and Lars Hestbjerg Hansen and Angela Collier and Bodil Lund Jacobsen and Jennifer Clare Schlundt and O H Karlstr{\"o}m},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 3},
  pages={985-91}
}
We have developed very efficient suicide functions for biological containment based on the lethal Escherichia coli relF gene. The suicide functions are placed in duplicate within a plasmid and arranged to prevent inactivation by deletion, recombination, and insertional inactivation. The efficiency of this concept was tested in a plasmid containment system that prevents transfer of plasmids to wild-type bacteria. Protection against plasmid transfer was assayed in test tubes and in rat intestine… CONTINUE READING
19 Citations
21 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 21 references

Ecology of plasmids and unique DNA sequences

  • S. B. Levy
  • 1986

Similar Papers

Loading similar papers…