Development and properties of HepG2 cells that constitutively express CYP2E1.

@article{Wu2008DevelopmentAP,
  title={Development and properties of HepG2 cells that constitutively express CYP2E1.},
  author={Defeng Wu and Arthur I. Cederbaum},
  journal={Methods in molecular biology},
  year={2008},
  volume={447},
  pages={
          137-50
        }
}
  • Defeng Wu, Arthur I. Cederbaum
  • Published in Methods in molecular biology 2008
  • Chemistry, Medicine
  • CYP2E1, a member of the cytochrome P450 family, is induced by ethanol. CYP2E1 activates many hepatotoxins to their reactive toxic intermediate form, and generates reactive oxygen species (ROS) during its catalytic cycle. Induction of CYP2E1 plays an important role in ethanol-induced oxidant stress and ethanol toxicity. To study the biochemical and toxicological properties of CYP2E1, our laboratory developed a HepG2 cell line which constitutively expresses the human CYP2E1 form. These cells… CONTINUE READING

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