Development and optimization of a cell-based neutralizing antibody assay using a sample pre-treatment step to eliminate serum interference.

@article{McCutcheon2010DevelopmentAO,
  title={Development and optimization of a cell-based neutralizing antibody assay using a sample pre-treatment step to eliminate serum interference.},
  author={Krista M. McCutcheon and Valerie E. Quarmby and An Song},
  journal={Journal of immunological methods},
  year={2010},
  volume={358 1-2},
  pages={35-45}
}
We developed a neutralizing antibody assay (NAb assay), based upon the complement-dependent cytotoxicity (CDC) activity of a monoclonal human IgG(1) therapeutic (IgT), to characterize anti-therapeutic antibodies (ATA) in autoimmune patient serum. Neutralizing antibodies (NAb) were measured by a decrease in the extent of CDC mediated by 50 ng/mL IgT, on a… CONTINUE READING