Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions.

@article{Calleri2007DevelopmentAI,
  title={Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions.},
  author={Enrica Calleri and Giorgio Marrubini and Gloria Brusotti and Gabriella Massolini and Gabriele Caccialanza},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2007},
  volume={44 2},
  pages={396-403}
}
The development and characterization of an anti-aflatoxin B1 (anti-AFB1) immunoaffinity monolithic disk is reported. Polyclonal anti-AFB1 was covalently immobilized in batch on an epoxy-activated monolithic Convective Interaction Media (CIM) disk (12 mm x 3 mm i.d.) by a one-step reaction via epoxy groups of the polymer surface. 0.96 mg of antibody were immobilized and the binding capacity of the CIM disk was determined by frontal analysis. The CIM disk was coupled through a switching valve to… CONTINUE READING

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