Development and implementation of split-GFP-based bimolecular fluorescence complementation (BiFC) assays in yeast.

@article{Barnard2008DevelopmentAI,
  title={Development and implementation of split-GFP-based bimolecular fluorescence complementation (BiFC) assays in yeast.},
  author={Emma Barnard and Neil V. McFerran and A. G. Trudgett and John A. Nelson and David J. Timson},
  journal={Biochemical Society transactions},
  year={2008},
  volume={36 Pt 3},
  pages={479-82}
}
BiFC (bimolecular fluorescence complementation) is a tool for investigating interactions between proteins. Non-fluorescent fragments of, for example, GFP (green fluorescent protein) are fused to the interacting partners. The interaction brings the fragments together, which then fold, reassemble and fluoresce. This process can be carried out in living cells and provides information both on the interaction and its subcellular location. We have developed a split-GFP-based BiFC assay for use in the… CONTINUE READING

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Detection of protein–protein interactions using protein-fragment complementation (PCA) assays

  • E. Barnard, N. V. McFerran, J. Nelson, D. J. Timson
  • Curr. Proteomics
  • 2007

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