Development and functional assessment of EST-derived 2RL-specific markers for 2BS.2RL translocations

@article{Lee2009DevelopmentAF,
  title={Development and functional assessment of EST-derived 2RL-specific markers for 2BS.2RL translocations},
  author={Tong-Geon Lee and Min Jeong Hong and Jerry W. Johnson and Daniel E. Bland and Dae Yeon Kim and Yong Weon Seo},
  journal={Theoretical and Applied Genetics},
  year={2009},
  volume={119},
  pages={663-673}
}
ESTs-derived markers are useful for comparative genomic analysis and can also serve as phenotype-linked functional markers. Here, we report the development of EST-derived 2RL-specific markers and the evaluation of the possibility of functional assessment of markers tagging 2RL, which carries Hessian fly resistance genes (loci). To identify transcripts specific to 2RL, unigene sequences in combination with wheat progenitor genomes were used. Total 275 contigs mapped to the long arms of… 
Development of gene-based markers for the identification of wheat-rye translocations possessing 1RS
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Through in silico analysis and primer validation, the authors were able to develop four 1RS-specific markers and these markers were confirmed with analysis involving 15 1RS translocation lines.
Development and Application of EST-Based Markers Specific for Chromosome Arms of Rye (Secale cereale L.)
  • H. Xu, D. Yin, +5 authors D. An
  • Biology, Medicine
    Cytogenetic and Genome Research
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TLDR
The results indicated that EST-based markers can effectively be used to detect corresponding rye chromosomes or chromosome arms introgressed into a wheat background, and hence to accelerate the utilization of rye genes in wheat breeding.
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Ten homoeologous markers specific to each short arm of chromosome 1 of common wheat and rye based on genome databases were developed and validated using wheat aneuploid and rye chromosome addition lines and recombinant inbred lines (RILs) were developed by crossing T1RS.1BL lines.
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A genome-wide analysis of transcripts in a 2BS.2RL wheat-rye translocation during Hessian fly infestation
TLDR
Diverse resistant NIL-specific transcripts including ethylene-responsive factor-like transcription factors and zinc-finger proteins were discovered and evidence of the enrichment of gene ontologies associated with hydrolase was found inresistant NIL during Hessian fly infestation.
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TLDR
A new wheat–rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White and was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement.
Identification of genome-specific transcripts in wheat–rye translocation lines
TLDR
Using the array developed, genome-specific transcripts in a wheat–rye translocation line are identified and deposited in the NCBI Gene Expression Omnibus (GEO) under accession number GSE58678.
Characterization of a Triticum aestivum–Dasypyrum villosum T2VS·2DL translocation line expressing a longer spike and more kernels traits
TLDR
Compared to its recurrent parent CS and three other translocation lines, the T2VS·2DL translocation line has longer spikes, more spikelets and more grains per spike in two season years, which suggested that the alien segment may carry yield-related genes of D. villosum.
Comparison of proteomes between wheat-rye translocations and their recurrent parents
TLDR
Protein spots found specifically in 2BS.2RL, but not in non-2RL translocations were identified as β-glucosidase and vacuolar ATP synthase subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic background.
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References

SHOWING 1-10 OF 35 REFERENCES
Development of AFLP and STS markers for identifying wheat-rye translocations possessing 2RL
TLDR
The data suggest that primer set SJ07 amplifies a “2RL-specific” fragment of diagnostic value in wheat-rye translocation line, and two STS primers out of twelve STS primer sets enabled the detection of polymorphisms between Coker 797 and NIL carrying 2RL.
Comparative mapping of DNA sequences in rye (Secalecereale L.) in relation to the rice genome
TLDR
An inventory was made of EST-derived markers with known genomic positions in rye, which were related with those in rice, providing improved insight into the status of the rye–rice genome relationships.
Development of Arm Specific RAPD Markers for Rye Chromosome 2R in Wheat
TLDR
To compensate for the lack of clearly identifiable cytological markers on chromosome 2R, random amplified polymorphic DNAs (RAPDs) were employed as markers and should be useful for the localization of translocation breakpoints in recombinant wheat-rye chromosomes.
Comparative DNA sequence analysis of mapped wheat ESTs reveals the complexity of genome relationships between rice and wheat
TLDR
There has been an abundance of rearrangements, insertions, deletions, and duplications eroding the wheat-rice genome relationship that may complicate the use of rice as a model for cross-species transfer of information in non-conserved regions.
A PCR assay for detection of a 2RL.2BS wheat-rye chromosome translocation.
TLDR
Oligonucleotide primers synthesized from published sequences derived from the R173 family of moderately repetitive rye DNA were used in the DNA polymerase chain reaction (PCR) to identify specific markers for 2RL, a wheat-rye chromosome translocation present in the wheat germplasm line Hamlet.
Approaching the self-incompatibility locus Z in rye (Secale cereale L.) via comparative genetics
TLDR
Given that the ubiquitination of proteins is emerging as a general mechanism involved in different SI systems of plants, TC116908 appears to be a promising target for further investigation with respect to its relation to the SI system of the grasses.
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes
TLDR
In the development of wheat EST-PCR markers capable of efficiently sorting products derived from homoeologous genes, it is important to design primers able to amplify products that include intron sequences with insertion/deletion polymorphisms.
Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly
TLDR
Tight linkage of the markers will provide a means of detecting H32 presence in marker-assisted selection and gene pyramiding as an effective strategy for extending durability of deployed resistance.
Hessian fly resistance gene H13 is mapped to a distal cluster of resistance genes in chromosome 6DS of wheat
TLDR
Marker analysis revealed that a very small intercalary chromosomal segment carrying H13 was transferred from the H13 donor parent to the wheat line Molly, a starting point toward the map-based cloning of H13.
Development of simple sequence repeat markers in rye (Secale cereale L.).
TLDR
Simple sequence repeats, also referred to as microsatellites, represent a PCR-based marker system that has been described in mammalian and plant genomes in recent years and are shown to be superior to other DNA markers with respect to their level of polymorphism in self-pollinating crop plants.
...
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