Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71

@inproceedings{Shi2011DevelopmentAE,
  title={Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71},
  author={Weifeng Shi and Kun Li and Yun Tao Ji and Qingbo Jiang and Mei Shi and Zuhuang Mi},
  booktitle={BMC infectious diseases},
  year={2011}
}
BACKGROUND Hand, foot, and mouth disease (HFMD) caused by enterovirus 71 (EV71) is very common in China. It is difficult to distinguish between EV71 and coxsackievirus A16 (CVA16) infections in clinical HFMD patients. Routine laboratory diagnosis of EV71 infection is time-consuming and requires expensive instruments. In this study, we have developed a one-step, single tube, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of EV71… CONTINUE READING
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