Development and evaluation of real-time PCR assays for the detection of the newly identified KI and WU polyomaviruses.

@article{Bialasiewicz2007DevelopmentAE,
  title={Development and evaluation of real-time PCR assays for the detection of the newly identified KI and WU polyomaviruses.},
  author={S Bialasiewicz and David Mark Whiley and Stephen Bernard Lambert and Allan B Gould and Michael D. Nissen and Theo P. Sloots},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2007},
  volume={40 1},
  pages={9-14}
}
BACKGROUND Recently, novel human polyomaviruses, KI (KIV) and WU (WUV) were described. Their role in human disease has not yet been determined. OBJECTIVES The aim of this study was to develop sensitive and specific assays for the detection of KIV and WUV. STUDY Two KIV (KI-A and KI-B) and three WUV (WU-A, WU-B and WU-C) real-time polymerase chain reaction (rtPCR) assays were developed and evaluated. Clinical sensitivities and specificities were determined by testing 200 respiratory… CONTINUE READING