Development and evaluation of immunoassay for zeranol in bovine urine

  title={Development and evaluation of immunoassay for zeranol in bovine urine},
  author={Yuan Liu and Cun-zhen Zhang and Xiangyang Yu and Zhiyuan Zhang and Xiao Zhang and Rong-rong Liu and Xian-jin Liu and Zhen-ming Gong},
  journal={Journal of Zhejiang University SCIENCE B},
A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1:5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation (CVs… CONTINUE READING