Development and evaluation of an immunomagnetic separation-ELISA for the detection of Staphylococcus aureus thermostable nuclease in composite milk.

@article{Yazdankhah1999DevelopmentAE,
  title={Development and evaluation of an immunomagnetic separation-ELISA for the detection of Staphylococcus aureus thermostable nuclease in composite milk.},
  author={Siamak P. Yazdankhah and Liv S\olver\od and Shane M Simonsen and Eric A. Olsen},
  journal={Veterinary microbiology},
  year={1999},
  volume={67 2},
  pages={113-25}
}
An ELISA method based on monodisperse magnetic beads was developed for the detection of Staphylococcus aureus thermostable nuclease (TNase) in composite milk, wherein S. aureus TNase is captured by magnetic beads coated with monoclonal antibodies directed against TNase and subsequently detected by an enzyme-labelled MAb against the same antigen. Sensitivity of the test was approximately 1 ng TNase, which corresponds to the amount of TNase produced and secreted by approximately 10(5) S. aureus… CONTINUE READING