Development and evaluation of a simple, direct, solid-phase radioimmunoassay of serum cortisol from readily available reagents.

@article{McConway1986DevelopmentAE,
  title={Development and evaluation of a simple, direct, solid-phase radioimmunoassay of serum cortisol from readily available reagents.},
  author={M G McConway and Robert Sedgwick Chapman},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1986},
  volume={158 1},
  pages={59-70}
}
A simple, rapid solid-phase radioimmunoassay for serum cortisol was developed using cortisol antibody distributed by the Scottish Antibody Production Unit and commercially available radioiodinated cortisol ligand. The assay involves a 1-h incubation at ambient temperature, using the antibody covalently linked by the easily performed carbonyldiimidazole method, to microcrystalline cellulose. A detailed comparison of the accepted 0.125 mol/l citrate, pH 4.0, and an alternative 0.1 mol/l phosphate… CONTINUE READING