Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens

@article{Callison2006DevelopmentAE,
  title={Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens},
  author={S. Callison and D. Hilt and T. O. Boynton and Brenda F Sample and R. Robison and D. Swayne and M. Jackwood},
  journal={Journal of Virological Methods},
  year={2006},
  volume={138},
  pages={60 - 65}
}
  • S. Callison, D. Hilt, +4 authors M. Jackwood
  • Published 2006
  • Medicine, Biology
  • Journal of Virological Methods
  • It is important to rapidly differentiate infectious bronchitis virus (IBV) from disease agents like highly pathogenic avian influenza virus and exotic Newcastle disease virus, which can be extremely similar in the early stages of their pathogenesis. [...] Key Result The assay amplifies a 143-bp product in the 5'-UTR of the IBV genome and has a limit of detection and quantification of 100 template copies per reaction.Expand Abstract
    220 Citations
    Investigation of Infectious Bronchitis Virus Strains by Real Time RT-PCR and S1 Gene Sequencing in Broilers
    • 1
    • Highly Influenced
    Development and application of an RT-PCR test for detecting avian nephritis virus
    • 25
    Development and Validation of RT‐PCR Tests for the Detection and S1 Genotyping of Infectious Bronchitis Virus and Other Closely Related Gammacoronaviruses Within Clinical Samples
    • 28

    References

    SHOWING 1-10 OF 16 REFERENCES
    Further development and use of a molecular serotype identification test for infectious bronchitis virus.
    • 85
    A monoclonal antibody blocking ELISA to detect serotype-specific infectious bronchitis virus antibodies.
    • 36
    Origin and evolution of Georgia 98 (GA98), a new serotype of avian infectious bronchitis virus.
    • 67
    Turkey coronavirus is more closely related to avian infectious bronchitis virus than to mammalian coronaviruses: A review
    • J. Guy
    • Biology, Medicine
    • Avian pathology : journal of the W.V.P.A
    • 2000
    • 69
    • PDF
    A Laboratory Manual for the Isolation and Identification of Avian Pathogens
    • 1,459