Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.

@article{Cole2009DevelopmentAE,
  title={Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.},
  author={Justin M Cole and Audrey N. Schuetz and Charles Ed Hill and Frederick S Nolte},
  journal={Journal of clinical microbiology},
  year={2009},
  volume={47 2},
  pages={322-6}
}
We developed a novel real-time PCR assay to detect Klebsiella pneumoniae carbapenemases (KPCs) and used this assay to screen clinical isolates of K. pneumoniae and Klebsiella oxytoca for the presence of bla(KPC) genes. The TaqMan real-time PCR assay amplified a 399-bp product from the bla(KPC) gene. The amplicon was designed so that the genes for isoenzymes KPC-1, -2, and -3 could be easily distinguished by subsequent restriction digestion of the amplicon with the enzymes BstNI and RsaI. The… CONTINUE READING