Development and evaluation of a rapid enzyme-immunoassay system for measurement of the urinary concentration of estrone-3-glucuronide in a female giant panda (Ailuropoda melanoleuca).

@article{Hama2008DevelopmentAE,
  title={Development and evaluation of a rapid enzyme-immunoassay system for measurement of the urinary concentration of estrone-3-glucuronide in a female giant panda (Ailuropoda melanoleuca).},
  author={Natsuki Hama and Hideyasu Kanemitsu and Kensuke Sakamoto and Yujiro Oyama and Tomas Javier Acosta and Osamu Ishikawa and Wang Pengyan and Kiyoshi Okuda},
  journal={The Journal of reproduction and development},
  year={2008},
  volume={54 4},
  pages={281-5}
}
To detect estrus for reproductive management, and to determine the relationship between urinary estrogen and estrous behavior, in a female giant panda, we developed and evaluated a rapid enzyme immunoassay (EIA) system for urinary Estrone-3-glucuronide (E1G) using commercial reagents. The developed EIA system took only around 3 hours, including all procedures to obtain a result. It indicated good reproducibility (intra-assay CV of 5.16%, interassay CV of 15.4%) and sensitivity (lowest standard… CONTINUE READING