Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species.

@article{Becker2004DevelopmentAE,
  title={Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species.},
  author={Karsten Becker and Dag Harmsen and Alexander Mellmann and Christian Meier and Peter Schumann and Georg Peters and Christof von Eiff},
  journal={Journal of clinical microbiology},
  year={2004},
  volume={42 11},
  pages={4988-95}
}
To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal… CONTINUE READING
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