Development and evaluation of a polymerase chain reaction (PCR) assay for the detection of Opisthorchis viverrini in fish.

@article{Parvathi2008DevelopmentAE,
  title={Development and evaluation of a polymerase chain reaction (PCR) assay for the detection of Opisthorchis viverrini in fish.},
  author={Ammini Parvathi and Kanasinakatte Rudrappa Umesha and Sanath Kumar and Paiboon Sithithaworn and I Karunasagar and Iddya Karunasagar},
  journal={Acta tropica},
  year={2008},
  volume={107 1},
  pages={13-6}
}
Human opisthorchiais caused by the liver fluke Opisthorchis viverrini is a major fish-borne trematode infection endemic in the Southeast Asian countries. The infection is acquired through consumption of raw fish harboring metacercariae of O. viverrini. Owing to potential risk of transmission of opisthorchiasis through fish trade, rapid and reliable detection methods have gained importance to ensure food safety. In the study described here, we report successful development and evaluation of a… CONTINUE READING