Development and evaluation of a multiplex PCR for differentiation of foot-and-mouth disease virus strains native to India.

@article{Giridharan2005DevelopmentAE,
  title={Development and evaluation of a multiplex PCR for differentiation of foot-and-mouth disease virus strains native to India.},
  author={Palani Giridharan and Divakar Hemadri and Chakradhar Tosh and Aniket Sanyal and Santanu Kumar Bandyopadhyay},
  journal={Journal of virological methods},
  year={2005},
  volume={126 1-2},
  pages={1-11}
}
A multiplex PCR (mPCR) for the differentiation of Indian FMDV serotypes, O, A, Asia 1 and C was developed and evaluated on 142 clinical and 39 cell culture samples. On the latter samples both the tests worked well with 100% efficiency, whereas on clinical samples mPCR had better efficiency than ELISA. The test was found to be specific for FMDV. The detection limit of the assay was varied among the serotypes; it was most sensitive on types A and Asia 1 and least sensitive on type C. The mPCR… CONTINUE READING
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