Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.

@article{Hoshino1998DevelopmentAE,
  title={Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.},
  author={Kiichi Hoshino and Shinji Yamasaki and Asish K. Mukhopadhyay and Shantanu Chakraborty and Anand Basu and Sujit Bhattacharya and Gopinath Balakrish Nair and Tadahito Shimada and Yoshifumi Takeda},
  journal={FEMS immunology and medical microbiology},
  year={1998},
  volume={20 3},
  pages={
          201-7
        }
}
A multiplex polymerase chain reaction assay was developed for concurrent detection of rfb sequences specific for the O1 and the O139 serogroups of Vibrio cholerae and for ctxA specific sequences. The multiplex PCR assay was found to be highly specific and sensitive and was capable of detecting 65 cfu and 200 cfu per assay of V. cholerae O1 and O139, respectively. Evaluation of the multiplex PCR assay using 121 stool samples from patients admitted to the Infectious Diseases Hospital, Calcutta… CONTINUE READING

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