Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts.

@article{Jansen2011DevelopmentAE,
  title={Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts.},
  author={Rogier R. Jansen and Janke Schinkel and Sylvie M. Koekkoek and Dasja Pajkrt and Marcel Beld and Menno D. T. de Jong and Richard Molenkamp},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2011},
  volume={51 3},
  pages={179-85}
}
BACKGROUND Multiplex real time PCR is increasingly used to diagnose respiratory viruses and has shown to be superior to traditional methods, like culture and antigen detection. However, comprehensive data on sensitivity, specificity and performance of the multiplex PCR compared to the single target PCR's is limited for most published respiratory multiplex real time PCR assays. OBJECTIVES Development and extensive analysis of an internally controlled multiplex real time rt-PCR for detection of… CONTINUE READING