Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis.

  title={Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis.},
  author={Pilar C Morata and Mar{\'i}a Isabel Queipo-Ortu{\~n}o and Jos{\'e} Mar{\'i}a Reguera and Miguel Angel Garc{\'i}a-Ordo{\~n}ez and Ana C{\'a}rdenas and Juan de Dios Colmenero},
  journal={Journal of clinical microbiology},
  volume={41 1},
In order to overcome some of the limitations of conventional microbiological techniques in the diagnosis of human brucellosis, a simple PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed. After amplification of a 223-bp sequence of a gene that codes for the synthesis of an immunogenetic membrane protein specific for the Brucella genus (BCSP31), the digoxigenin-labeled amplified product was hybridized with a biotinylated capture probe which was complementary to the inner part of the… CONTINUE READING


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