Development and characterization of the NanoOrange protein quantitation assay: a fluorescence-based assay of proteins in solution.

@article{Jones2003DevelopmentAC,
  title={Development and characterization of the NanoOrange protein quantitation assay: a fluorescence-based assay of proteins in solution.},
  author={Laurie J Jones and Richard P. Haugland and Victoria L. Singer},
  journal={BioTechniques},
  year={2003},
  volume={34 4},
  pages={850-4, 856, 858 passim}
}
We developed a sensitive fluorescence assay for the quantitation of proteins in solution using the NanoOrange reagent, a merocyanine dye that produces a large increase in fluorescence quantum yield upon interaction with detergent-coated proteins. The NanoOrange assay allowed for the detection of 10 ng/mL to 10 micrograms/mL protein with a standard fluorometer, offering a broad, dynamic quantitation range and improved sensitivity relative to absorption-based protein solution assays. The protein… CONTINUE READING