Development and characterization of new cell line BPH22 from midgut epithelial cells of Poekilocerus pictus (Fabricius, 1775)

@article{Kharat2010DevelopmentAC,
  title={Development and characterization of new cell line BPH22 from midgut epithelial cells of Poekilocerus pictus (Fabricius, 1775)},
  author={Kiran R. Kharat and Madhavi V. Sawant and Shiney Mary Peter and Bhagyashree P. Hardikar},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={2010},
  volume={46},
  pages={824-827}
}
Poekilocerus pictus is a painted grasshopper, which feeds on Calotropis sp. containing the cardiac glycosides. A new cell line BPH22 is developed from midgut of P. pictus to study its unique physiology and biochemistry. Initially, the Graces insect medium is used with 10% (v/v) fetal bovine serum. After four passages, the serum quantity was reduced up to 0%.The lag phase in growth curve was 5 d and log phase is up to 10–11 d. The addition of Calotropis extract in Graces medium enhanced the… CONTINUE READING
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References

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Showing 1-10 of 12 references

Development and characterization of insect cell lines

  • E LynnD.
  • Cytotechnology
  • 1996

Insect midgut epithelium in vitro : an insect stem cell system

  • M. Loeb, R Hakim
  • J . Insect Physiol .
  • 1996
2 Excerpts

Proliferation and differentiation of midgut epithelial cells from Manduca sexta, in vitro

  • S. Sadrud-din, R. Hakim, M. Loeb
  • Invertebr. Reprod. Dev
  • 1994
1 Excerpt

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