Development and characterization of an in vitro ovulation model using mouse ovarian follicles.

@article{Rose1999DevelopmentAC,
  title={Development and characterization of an in vitro ovulation model using mouse ovarian follicles.},
  author={Ursula M Rose and Rob G. J. M. Hanssen and Helenius J. Kloosterboer},
  journal={Biology of reproduction},
  year={1999},
  volume={61 2},
  pages={503-11}
}
To investigate ovulation, an in vitro model with cultured mouse follicles was developed and compared with an in vivo ovulation model. In this model, secondary follicles were grown in vitro with immature mouse serum (5%) and recombinant human FSH. Addition of ascorbic acid and selenium to the medium increased follicular survival (from 29% to 86%) and resulted in the development of healthy preovulatory follicles (> 400 microm) producing estradiol. Depending on the starting size of the follicles… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
31 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 31 extracted citations

Similar Papers

Loading similar papers…