Development and characterization of a whole-cell bioluminescent sensor for bioavailable middle-chain alkanes in contaminated groundwater samples.

@article{Sticher1997DevelopmentAC,
  title={Development and characterization of a whole-cell bioluminescent sensor for bioavailable middle-chain alkanes in contaminated groundwater samples.},
  author={P Sticher and Marco C M Jaspers and Konrad Stemmler and Hauke Harms and Alexander J. B. Zehnder and Jan Roelof van der Meer},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 10},
  pages={4053-60}
}
A microbial whole-cell biosensor was developed, and its potential to measure water-dissolved concentrations of middle-chain-length alkanes and some related compounds by bioluminescence was characterized. The biosensor strain Escherichia coli DH5 alpha(pGEc74, pJAMA7) carried the regulatory gene alkS from Pseudomonas oleovorans and a transcriptional fusion of PalkB from the same strain with the promoterless luciferase luxAB genes from Vibrio harveyi on two separately introduced plasmids. In… CONTINUE READING
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