Development and characterization of a cell line for large-scale, serum-free production of recombinant adeno-associated viral vectors.

@article{Farson2004DevelopmentAC,
  title={Development and characterization of a cell line for large-scale, serum-free production of recombinant adeno-associated viral vectors.},
  author={Deborah A. Farson and Thomas C. Harding and Lucy Tao and Jun Liu and Sandra Powell and Vishalini Vimal and Satyasri Yendluri and Kathryn E. Koprivnikar and Kenneth Wai Kheong Ho and Christopher G. Twitty and Paul J. Husak and Andy Yu-Lun Lin and Richard O. Snyder and Brian A. Donahue},
  journal={The journal of gene medicine},
  year={2004},
  volume={6 12},
  pages={1369-81}
}
BACKGROUND One of the major limitations to the use of adeno-associated virus (AAV) vectors for gene therapy has been the difficulty in producing enough vector to supply a clinical trial. More than 20 000 roller bottles may be required to generate AAV by the traditional transient transfection process to treat 50 patients. A scalable AAV producer cell line… CONTINUE READING