Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1

@article{Yang2012DevelopmentAA,
  title={Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1},
  author={Limin Yang and Jing zhou Li and Yuhai Bi and Lei Xu and Wenjun Liu},
  journal={Virus Genes},
  year={2012},
  volume={45},
  pages={585-589}
}
We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Duck hepatitis A virus type 1 (DHAV-1). The amplification could be finished in 1 h under isothermal conditions at 63 °C by employing a set of four primers targeting the 2C gene of DHAV-1. The RT-LAMP assay showed higher sensitivity than the RT-PCR with a detection limit of 0.1 ELD50 0.1 ml−1 of DHAV-1. The RT-LAMP assay was highly specific; no cross-reactivity was observed… CONTINUE READING