Development and application of a loop-mediated isothermal amplification assay for rapid identification of aflatoxigenic molds and their detection in food samples.

@article{Luo2012DevelopmentAA,
  title={Development and application of a loop-mediated isothermal amplification assay for rapid identification of aflatoxigenic molds and their detection in food samples.},
  author={Jie Luo and Rudi F. Vogel and Ludwig Niessen},
  journal={International journal of food microbiology},
  year={2012},
  volume={159 3},
  pages={
          214-24
        }
}
Aflatoxins are the most thoroughly studied mycotoxins. They are produced by several members of the genus Aspergillus in section Flavi with Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius being frequently isolated from contaminated food sources. In this work, we describe the development and evaluation of loop-mediated isothermal amplification (LAMP) assays for rapid detection of the three species in separate analyses. The acl1-gene of A. flavus and amy1-genes of A. nomius and… Expand
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TLDR
The LAMP assays described in this study have been demonstrated to be a specific, sensitive and easy to use tool for the survey of Brazil nuts for contaminations with potential aflatoxin-producing A. nomius and A. flavus in low tech environments where resources may be limited. Expand
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Detection, identification, and quantification of aflatoxin producing fungi in food raw materials using loop-mediated isothermal amplification (LAMP) assays
TLDR
It has been shown that the loop-mediated isothermal amplification (LAMP) technology is well suited to estimate aflatoxin contamination in samples, particularly suitable for use under low-tech conditions. Expand
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