Development and application of a heterologous radioimmunoassay for ovine follicle-stimulating hormone.

@article{McNeilly1976DevelopmentAA,
  title={Development and application of a heterologous radioimmunoassay for ovine follicle-stimulating hormone.},
  author={Judy R McNeilly and Alan McNeilly and John S Walton and Frederic Cunningham},
  journal={The Journal of endocrinology},
  year={1976},
  volume={70 1},
  pages={69-79}
}
A highly specific and sensitive heterologous double antibody radioimmunoassay for ovine follicle-stimulating hormone (oFSH) is described in detail. The assay using a rabbit antiserum to human FSH and either 125I-labelled rat FSH or 125I-labelled oFSH as tracer is specific for FSH. A maximum cross-reaction (B/Bo = 50%) of 0-1% was observed with other ovine, rat or human pituitary hormones or human chorionic gonadotrophin. Serum levels of oFSH in samples collected daily throughout the oestrous… CONTINUE READING