Development and application of a broadly reactive real-time reverse transcription-PCR assay for detection of murine noroviruses.

@article{Kitajima2010DevelopmentAA,
  title={Development and application of a broadly reactive real-time reverse transcription-PCR assay for detection of murine noroviruses.},
  author={Masaaki Kitajima and Tomoichiro Oka and Hirotaka Takagi and Yukinobu Tohya and Hiroyuki Katayama and Naokazu Takeda and Kazuhiko Katayama},
  journal={Journal of virological methods},
  year={2010},
  volume={169 2},
  pages={269-73}
}
Murine norovirus (MNV) is a viral agent newly identified in laboratory mice and a large number of genetically diverse MNV strains have been reported to date. A broadly reactive TaqMan-based real-time reverse transcription (RT)-polymerase chain reaction (PCR) assay was developed for MNVs. Novel primers and a TaqMan MGB probe were designed targeting highly conserved sequences among MNV strains, which are located in the open reading frames 1 (ORF1)-ORF2 junction region. The quantitative range of… CONTINUE READING
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