Development and application of a most-probable-number-pcr assay to quantify flagellate populations in soil samples.

@article{Fredslund2001DevelopmentAA,
  title={Development and application of a most-probable-number-pcr assay to quantify flagellate populations in soil samples.},
  author={L Fredslund and Flemming Ekelund and Carsten Jacobsen and Kaare Johnsen},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 4},
  pages={1613-8}
}
This paper reports on the first successful molecular detection and quantification of soil protozoa. Quantification of heterotrophic flagellates and naked amoebae in soil has traditionally relied on dilution culturing techniques, followed by most-probable-number (MPN) calculations. Such methods are biased by differences in the culturability of soil protozoa and are unable to quantify specific taxonomic groups, and the results are highly dependent on the choice of media and the skills of the… CONTINUE READING