Development and application of a PCR-based method including an internal control for diagnosis of congenital cytomegalovirus infection.

@article{Jones2000DevelopmentAA,
  title={Development and application of a PCR-based method including an internal control for diagnosis of congenital cytomegalovirus infection.},
  author={Ronald N. Jones and Michael L Neale and Bradley Beattie and Diana Westmoreland and Julie D. Fox},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 1},
  pages={1-6}
}
Cytomegalovirus (CMV) is the most common cause of congenital infection in the developed world. We have designed and evaluated an assay that includes an internal control for amplification and detection of CMV DNA in amniotic fluid and neonatal urine samples. We present data on the use of this assay in the diagnosis of congenital CMV infection. A total of 145 amniotic and fetal fluid samples were examined by this assay; 83 were from healthy pregnant women and 62 were from women who were being… CONTINUE READING