Development and analysis of a tick-borne encephalitis virus infectious clone using a novel and rapid strategy.

@article{Gritsun1998DevelopmentAA,
  title={Development and analysis of a tick-borne encephalitis virus infectious clone using a novel and rapid strategy.},
  author={Tamara Sergeevna Gritsun and Ernest Andrew Gould},
  journal={Journal of virological methods},
  year={1998},
  volume={76 1-2},
  pages={109-20}
}
In less than 1 month we have constructed an infectious clone of attenuated tick-borne encephalitis virus (strain Vasilchenko) from 100 microl of unpurified virus suspension using long high fidelity PCR and a modified bacterial cloning system. Optimization of the 3' antisense primer concentration was essential to achieve PCR synthesis of an 11 kb cDNA copy of RNA from infectious virus. A novel system utilising two antisense primers, a 14-mer for reverse transcription and a 35-mer for long PCR… CONTINUE READING
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