Development and Validation of an Rnaseq NGS Assay to Comprehensively Detect Clinically Relevant ALL Gene Fusions

@article{Sitlani2017DevelopmentAV,
  title={Development and Validation of an Rnaseq NGS Assay to Comprehensively Detect Clinically Relevant ALL Gene Fusions},
  author={Parth Sitlani and Maya Panjikaran and Dana Weiner and Claire Orosco and Durga Prasad Cherukuri and Lauryn Keeler and Matthew M. Dedek and Yuchao Xia and Armin Graber and Christopher J. Scott and Jeffrey David Falk},
  journal={Blood},
  year={2017},
  volume={130},
  pages={1431-1431}
}
Background Recent advances in molecular technologies have identified a subtype of B-progenitor acute lymphoblastic leukemia (B-ALL) with a Philadelphia chromosome-like (Ph-like or BCR-ABL1-like) gene expression profile without the BCR-ABL1 fusion. The subtypes containing Ph-like fusions are considered the more aggressive subtype. Although FISH and RT-PCR assays can be used to detect these fusions, these technologies are labor intensive and low-throughput in nature. An optimal solution is to…