Development and Validation of an Enzyme Immunoassay for Fecal Dehydroepiandrosterone Sulfate in Japanese Macaques (Macaca fuscata)

  title={Development and Validation of an Enzyme Immunoassay for Fecal Dehydroepiandrosterone Sulfate in Japanese Macaques (Macaca fuscata)},
  author={Rafaela S. C. Takeshita and Fred B. Bercovitch and Michael Alan Huffman and Kodzue Kinoshita},
  journal={International Journal of Primatology},
Measuring hormonal profiles is important in monitoring stress, physical fitness, and reproductive status in primates. Noninvasive methods have been used to measure several steroid hormones in primates without causing them stress. However, few studies have used feces or urine to measure dehydroepiandrosterone sulfate (DHEAS), an important precursor of sex steroids that has been studied as a biomarker of aging, pregnancy, and stress in humans and nonhuman primates. We developed an enzyme… 
Validation of an enzyme immunoassay for measurement of fecal dehydroepiandrosterone sulfate in gibbons and siamangs.
An enzyme immunoassay (EIA) described has high sensitivity and it is suitable for fecal DHEAS measurement in gibbons and siamangs, with a potential to be applied to other species.
The effects of transport stress on the behaviour and adrenocortical activity of the black-and-white ruffed lemur (Varecia variegata)
The results of both behaviour and adrenocortical activity analysis suggest that despite some presented changes in the monitored indices prior and after transport, the transportation under the studied conditions presented only a mild stressor with limited behavioural and glucocorticoid responses.
Stress, Well-Being and Reproductive Success.
The potential for stress to disrupt, and sometimes facilitate reproduction, including the key role that glucocorticoids play is discussed, as well as a number of physiological biomarkers, which have the potential to assess well-being and the role of stress on reproduction.
  • Abstracts in Anthropology
  • 2019


Comparison of different enzymeimmunoassays for assessment of adrenocortical activity in primates based on fecal analysis
The data suggest that the reliability of a given fecal glucocorticoid assay in reflecting activity of the HPA axis in primates clearly depends on the species in question, and group‐specific assays have a high potential for cross‐species application.
Dehydroepiandrosterone sulfate levels reflect endogenous luteinizing hormone production and response to human chorionic gonadotropin challenge in older female macaque (Macaca fascicularis)
A positive adrenal androgen response to LH/chorionic gonadotropin in older female higher primates is demonstrated and a mechanism for the rise in adrenalandrogen production during the menopausal transition in women is suggested.
Dehydroepiandrosterone-sulfate as a biomarker of senescence in male non-human primates
Pharmacokinetics of oral dehydroepiandrosterone (DHEA) in the ovariectomised cynomolgus monkey
ACTH stimulation tests and plasma dehydroepiandrosterone sulfate levels in women with hirsutism.
Environmental, biological, and social factors influencing fecal adrenal steroid concentrations in female Japanese macaques (Macaca fuscata)
Results revealed that both fGC and fecal DHEAS concentrations are higher in females housed indoors in single cages than in those living outdoors in social groups, and fGC concentrations were higher in the cycling females during the mating season than the lactating females in the birth season.
Dehydroepiandrosterone (DHEA), DHEA sulfate, and aging: contribution of the DHEAge Study to a sociobiomedical issue.
A number of biological indices confirmed the lack of harmful consequences of this 50 mg/day DHEA administration over one year, indicating that this kind of replacement therapy normalized some effects of aging, but does not create "supermen/women" (doping).
Measuring Fecal Glucocorticoid Metabolites in Mammals and Birds: The Importance of Validation
  • C. Touma, R. Palme
  • Biology, Environmental Science
    Annals of the New York Academy of Sciences
  • 2005
Comprehensive information on the animals' biology and stress physiology should be carefully taken into account to ensure that the measurement of fecal GCMs can be used as a powerful tool to assess adrenocortical activity in diverse investigations on laboratory, companion, farm, zoo, and wild animals.