Development and Validation of a HPLC-DAD Separation Method for Determination of Bioactive Antrocin in Medicinal Mushroom Antrodia Camphorata

  title={Development and Validation of a HPLC-DAD Separation Method for Determination of Bioactive Antrocin in Medicinal Mushroom Antrodia Camphorata},
  author={Madamanchi Geethangili and Yerra Koteswara Rao and Yew-Min Tzeng},
  journal={International Journal of Applied Science and Engineering},
Mushrooms are a food with high nutritional value. Antrodia camphorata (AC), is a medicinal mushroom being widely used as food dietary supplement for cancer prevention. The sesquiterpene lactone, antrocin reported as a novel dual Akt and mTOR inhibitor in metastatic breast cancer cells and, is the most potent among more than one hundred secondary metabolites isolated from AC. For the first time, this study developed and validated a simple high-performance liquid chromatography coupled with diode… 

Figures and Tables from this paper

Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-β/c-Fos-MMP-7 Pathways

It is demonstrated that the anti-invasive effect of antcin-H in human renal carcinoma 786-0 cells might be at least in part by abrogating focal adhesion complex and lamellipodium formation through inhibiting the Src/FAK-paxillin signaling pathways and decreasing MMP-7 expression through suppressing the ERK1/2-AP-1/c-Fos and C/EBP-β signaling axis.

Antibacterial properties of wild edible and non-edible mushrooms found in Zimbabwe

The positive results of screening local mushrooms for antibacterial activity forms the basis for further phytochemical studies and development of antimicrobial agents against common human bacterial and fungal infections.

Multiomics Identification of Potential Targets for Alzheimer Disease and Antrocin as a Therapeutic Candidate

A new molecular signature for AD parthenogenesis and antrocin as a potential therapeutic agent is suggested and good predictions for ADMET properties, drug-likeness, adherence to Lipinskís rules, and no alerts for pan-assay interference compounds are revealed.

Corroborative assessment of mushroom as the graceful ageing and lifespan promoting agent

Assessment of edible-medicinal mushrooms with respect to their anti-ageing and lifespan promoting capabilities, stand them in ideal stead as a therapeutic agent for the global ever-mushrooming aged population.

Evaluation of HPLC and MEKC methods for the analysis of lipopeptide antibiotic iturin A produced by Bacillus amyloliquefaciens

Two methods, high-performance liquid chromatography (HPLC) and micellar elec- trokinetic chromatography (MEKC), for analysis of multicomponent lipopeptide antibiotic iturin A are described. After

Review of Pharmacological Effects of Antrodia camphorata and Its Bioactive Compounds

It is concluded that A. camphorata can be considered as an efficient alternative phytotherapeutic agent or a synergizer in the treatment of cancer and other immune-related diseases, however, clinical trails of human on A.camphorata extracts are limited and those of pure compounds are absent.

Review of biological and pharmacological activities of the endemic Taiwanese bitter medicinal mushroom, Antrodia camphorata (M. Zang et C. H. Su) Sh. H. Wu et al. (higher Basidiomycetes).

This review summarizes the latest published results from A.camphorata research, focusing on the biological and pharmacological activities of the crude extract and known constituents of A. camphorata.

Identification of antrocin from Antrodia camphorata as a selective and novel class of small molecule inhibitor of Akt/mTOR signaling in metastatic breast cancer MDA-MB-231 cells.

It is reported for the first time that a small molecule, antrocin, from Antrodia camphorata was a potent antagonist in various cancer types, being highest in metastatic breast cancer MDA-MB-231 cells (MMCs) with an IC(50) value of 0.6 μM.

Cytotoxic Effect of trans-Cinnamaldehyde from Cinnamomum osmophloeum Leaves on Human Cancer Cell Lines

Results suggest that TCA may be a useful chemotherapeutic agent for cancer treatment in human by cell cycle analysis and inhibitory effects of TCA against selected human cancer cell lines.

Current findings, future trends, and unsolved problems in studies of medicinal mushrooms

  • S. Wasser
  • Biology, Medicine
    Applied Microbiology and Biotechnology
  • 2010
Several of the mushroom polysaccharide compounds have proceeded through phases I, II, and III clinical trials and are used extensively and successfully in Asia to treat various cancers and other diseases.

The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health.

A pyramid model for mushroom uses (industries), as food, dietary supplements (tonic), and medicine is presented and many critically important unsolved problems in the future development of medicinal mushroom science in the 21st century are drawn attention.

Medicinal mushrooms for glycemic control in diabetes mellitus: history, current status, future perspectives, and unsolved problems (review).

  • H. LoS. Wasser
  • Medicine
    International journal of medicinal mushrooms
  • 2011
An overview of the multiple aspects of diabetes mellitus and the efficacy and mechanism of medicinal mushrooms for glucose control in diabetes, including the inhibition of glucose absorption, protection of beta-cell damage, increase of insulin release, enhancement of antioxidant defense, attenuation of inflammation, modulation of carbohydrate metabolism pathway, and regulation of insulin-dependent and insulin-independent signaling pathways are presented.