Development and Validation of High Performance Thin Layer Chromatographic Method for Estimation of Brimonidine Tartrate as Bulk Drug and in Ophthalmic Solutions

@inproceedings{Anand2003DevelopmentAV,
  title={Development and Validation of High Performance Thin Layer Chromatographic Method for Estimation of Brimonidine Tartrate as Bulk Drug and in Ophthalmic Solutions},
  author={Mahajan Anand and Athensia Fonseca and Gandhi Santosh and Deshpande Padmanabh},
  year={2003}
}
A simple, specific and precise high performance thin layer chromatographic method was developed and validated for estimation of Brimonidine tartrate as bulk drug and in ophthalmic solutions. The chromatographic development was carried out on precoated silica gel 60 F254 aluminium plates using mixture of Methanol: Ammonia (8:0.2 v/v) as mobile phase and densitometric evaluation of band was carried out at 250 nm using Camag TLC Scanner-3 with win CAT 1.4.3 version software. The Rf value of drug… CONTINUE READING