Development, characterization, and evaluation of a fusion protein of a novel glucagon-like peptide-1 (GLP-1) analog and human serum albumin in Pichia pastoris.

@article{Gao2009DevelopmentCA,
  title={Development, characterization, and evaluation of a fusion protein of a novel glucagon-like peptide-1 (GLP-1) analog and human serum albumin in Pichia pastoris.},
  author={Zhihui Gao and Gang Bai and Jiaqi Chen and Qi Zhang and Pengwei Pan and Fang Bai and Peng Geng},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2009},
  volume={73 3},
  pages={688-94}
}
Glucagon-like peptide-1 (GLP-1) has considerable potential as a possible therapeutic agent for type-2 diabetes. Unfortunately, this glucoincretin is short lived due to degradation by dipeptidyl-peptidase IV and rapid clearance by renal filtration. In this study, we attempted to extend GLP-1 action through the attachment of a lysine residue at the N-terminal of GLP-1 (named KGLP-1), and to make a fusion protein with human serum albumin (HSA) in Pichia pastoris. The protein, designated KGLP-1/HSA… CONTINUE READING