Developing rice embryo proteomics reveals essential role for embryonic proteins in regulation of seed germination.

@article{Kim2009DevelopingRE,
  title={Developing rice embryo proteomics reveals essential role for embryonic proteins in regulation of seed germination.},
  author={Sun Tae Kim and Yiming Wang and Sun Young Kang and Sang Gon Kim and Randeep Rakwal and Yong Chul Kim and Kyu Young Kang},
  journal={Journal of proteome research},
  year={2009},
  volume={8 7},
  pages={3598-605}
}
Two-dimensional gel electrophoresis (2-DGE) in conjunction with mass spectrometry was utilized to unravel the changes in embryo proteins of geminating rice seeds. For this purpose, the embryos were dissected at 1 and 2 days after imbibition and germination of mature dry seeds. Proteins were extracted and their expression patterns were analyzed by large-format 2-DGE. A total of 642 silver nitrate stained protein spots were detected on 2-D gels, subjected to image analysis using the ImageMaster 6… CONTINUE READING