Developing markers for multilocus phylogenetics in non-model organisms: A test case with turtles.

@article{Thomson2008DevelopingMF,
  title={Developing markers for multilocus phylogenetics in non-model organisms: A test case with turtles.},
  author={R. C. Thomson and Andrew M. Shedlock and Scott V Edwards and Howard Bradley Shaffer},
  journal={Molecular phylogenetics and evolution},
  year={2008},
  volume={49 2},
  pages={514-25}
}
We present a strategy for phylogenetic marker development in non-model systems. Rather than using the traditional approach of comparing distantly related taxa to develop conserved primers for unknown species, we explore an alternative strategy that builds primers directly from a single, relatively well characterized species and applies those primers to increasingly distantly related taxa. We develop and test our protocol with turtles. Using a single BAC end-sequence library consisting of 3461… CONTINUE READING