Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production

@inproceedings{Cui2020DevelopingAC,
  title={Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production},
  author={Jingxuan Cui and D. Stevenson and Travis C. Korosh and Daniel Amador-Noguez and D. Olson and L. Lynd},
  booktitle={Frontiers in Energy Research},
  year={2020}
}
The cellulolytic bacterium Clostridium thermocellum is a promising candidate for lignocellulosic biofuel production; however, ethanol titer needs to be improved for commercialization. To understand the factors limiting ethanol titer in C. thermocellum, we developed a cell-free extract reaction (CFER) system. We demonstrated that 15 mM cellobiose could be converted, in vitro, to 25 mM ethanol and that this reaction functions both at thermophilic (55°C) and mesophilic (37°C) temperatures… Expand
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