Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology

  title={Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology},
  author={Long Liu and Yanfeng Liu and Hyun‐dong Shin and Rachel Chen and Nam Sun Wang and Jianghua Li and Guocheng Du and Jian Chen},
  journal={Applied Microbiology and Biotechnology},
  pages={6113 - 6127}
Increasing concerns over limited petroleum resources and associated environmental problems are motivating the development of efficient cell factories to produce chemicals, fuels, and materials from renewable resources in an environmentally sustainable economical manner. Bacillus spp., the best characterized Gram-positive bacteria, possesses unique advantages as a host for producing microbial enzymes and industrially important biochemicals. With appropriate modifications to heterologous protein… 

Engineering Bacillus subtilis Cells as Factories: Enzyme Secretion and Value-added Chemical Production

The versatile traits of B. subtilis, combined with multi-omics approaches and rapidly developing technologies for genome engineering and high-throughput screening enable this bacterium’s metabolic circuits to deliver compounds that are needed for a green and sustainable society as well as a healthy population.

Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

The progress in the development of B. subtilis as a universal platform to overproduce heterologous diverse high-value enzymes is reviewed and its ability to serve as a cellular tool was briefly recapitulated and reviewed.

Reduced and Minimal Cell Factories in Bioprocesses: Towards a Streamlined Chassis

  • M. ZieglerR. Takors
  • Biology, Engineering
    Minimal Cells: Design, Construction, Biotechnological Applications
  • 2019
The theoretical benefits of genome reduction form the basis of target selection and the current limits of beneficial genome reduction and potential future developments in both prokaryotic and eukaryotic systems are considered.

Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer

A Production Strain Blueprinting strategy to develop new production systems in a reduced time lapse in contrast to a development from scratch is suggested and a remarkable potential of B. pumilus to be the basis for a next generation production host is exhibited.

Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis.

This work reviews the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperatureinducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection and introduces the latest discoveries of Tat-related complex structures and functions.

Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.

This chapter illustrates the enormous possibilities offered by these groups of eukaryotic organisms for the overexpression of individual genes, or in the coexpression of multiples genes.

Stabilizing displayed proteins on vegetative Bacillus subtilis cells

Strain BRB08 is well suited for stably displaying reporter protein, as genetic removal of its extracellular and cell wall-associated proteases leads to the highest levels of surface-accumulated Cel8A-LysM without causing secretion stress or impairing growth.



Developments in the use of Bacillus species for industrial production.

Developments from the understanding of the complex protein translocation machinery of Gram-positive bacteria should allow the resolution of current secretion challenges and make Bacillus species preeminent hosts for heterologous protein production.

Bacillus subtilis: from soil bacterium to super-secreting cell factory

The engineering of B. subtilis into a next-generation super-secreting cell factory requires combined Systems and Synthetic Biology approaches and can be optimized from the single molecule to the network level while, at the same time, taking into account the balanced use of cellular resources.

One‐step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges

Rec recombinant cellulolytic B. subtilis strains would be a potential platform for biocommodity production from nonfood biomass and the opportunities and challenges are discussed.

Engineering microbial biofuel tolerance and export using efflux pumps

This work used bioinformatics to generate a list of all efflux pumps from sequenced bacterial genomes and prioritized a subset of targets for cloning, and efficiently distinguished pumps that improved survival and identified pumps that restored growth in the presence of biofuel.

Manufacturing Molecules Through Metabolic Engineering

Whereas existing production routes use well-known, safe, industrial microorganisms, future production schemes may include designer cells that are tailor-made for the desired chemical and production process.

Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis

  • T. MorimotoR. Kadoya N. Ogasawara
  • Biology, Engineering
    DNA research : an international journal for rapid publication of reports on genes and genomes
  • 2008
This is the first report demonstrating that genome reduction actually contributes to the creation of bacterial cells with a practical application in industry and further systematic analysis of changes in the transcriptional regulatory network of MGB874 cells in relation to protein productivity should facilitate the generation of improved B. subtilis cells as hosts of industrial protein production.

Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation.

A novel two-promoter system which can be used to independently control the expression levels of two gene cassettes over a large dynamic range is presented and lays the foundation for high level heterologous production of isoprenoids in Bacillus subtilis.

Combined Effect of Improved Cell Yield and Increased Specific Productivity Enhances Recombinant Enzyme Production in Genome-Reduced Bacillus subtilis Strain MGB874

It is reported that deletion of the rocDEF-rocR region, which is involved in arginine degradation, contributes to enhanced enzyme production in strain MGB874, a novel Bacillus subtilis strain derived from strain 168 that exhibits enhanced production of recombinant enzymes.

From Microbial Differentiation to Ribosome Engineering

  • K. Ochi
  • Biology
    Bioscience, biotechnology, and biochemistry
  • 2007
It is found that morphological differentiation results from a decrease in the pool of GTP, whereas physiological differentiationresults from a more direct function of ppGpp, and the novel concept of “ribosome engineering” is developed, focusing on activation of dormant genes to elicit cellular function fully.