Deuterium magic angle spinning studies of substrates bound to cytochrome P450.

@article{Lee1999DeuteriumMA,
  title={Deuterium magic angle spinning studies of substrates bound to cytochrome P450.},
  author={Homin K. Lee and Paul R. Ortiz de Montellano and Ann E McDermott},
  journal={Biochemistry},
  year={1999},
  volume={38 33},
  pages={10808-13}
}
We report solid-state deuterium magic angle spinning NMR spectra of perdeuterated adamantane bound to the active site of microcrystalline cytochrome P450cam (CP450cam) in its resting state. CP450cam contains a high-spin ferric (Fe3+) heme in the resting state; the isotropic shift was displaced from the diamagnetic value and varied with temperature consistent with Curie-law dependence. A nondeuterated competitive tighter binding ligand, camphor, was used to displace the adamantane-bound species… CONTINUE READING