Deuterium-exchange metabolomics identifies N-methyl lyso phosphatidylethanolamines as abundant lipids in acidophilic mixed microbial communities

Abstract

Natural microbial communities are extremely diverse and contain uncharacterized but functionally important small molecules. By coupling a deuterium (D) labeling technique to high mass accuracy untargeted liquid chromatography-electrospray ionization-mass spectrometry (LC–ESI–MS) metabolomic analysis, we found that natural acidophilic microbial biofilms dominated by bacteria of the genus Leptospirillum contained unusual lyso phosphatidylethanolamine (PE) lipids in high abundance (more than 10 nmol/mg of dry biomass). The unusual polar head group structure of these lipids is similar to lipids found in phylogenetically unrelated acidophilic chemoautolithotrophs and may be related to the affinity of these lipids for iron and calcium ions. Correlations of lyso phospholipid and proteome abundance patterns suggest a link between the lyso phospholipids and the UBA-type substrain of Leptospirillum group II. By combining untargeted metabolomics with D exchange we demonstrate the ability to identify cryptic but biologically functional small molecules in mixed microbial communities.

DOI: 10.1007/s11306-011-0344-x

Extracted Key Phrases

6 Figures and Tables

Statistics

010020020132014201520162017
Citations per Year

67 Citations

Semantic Scholar estimates that this publication has 67 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Fischer2011DeuteriumexchangeMI, title={Deuterium-exchange metabolomics identifies N-methyl lyso phosphatidylethanolamines as abundant lipids in acidophilic mixed microbial communities}, author={Curt Fischer and Paul Wilmes and Benjamin P. Bowen and Trent R. Northen and Jillian F. Banfield}, journal={Metabolomics}, year={2011}, volume={8}, pages={566-578} }