Deuterium NMR investigation of ether- and ester-linked phosphatidylcholine bilayers.

@article{Ruocco1985DeuteriumNI,
  title={Deuterium NMR investigation of ether- and ester-linked phosphatidylcholine bilayers.},
  author={Martin J. Ruocco and Alexandros Makriyannis and David J. Siminovitch and Robert G. Griffin},
  journal={Biochemistry},
  year={1985},
  volume={24 18},
  pages={
          4844-51
        }
}
Deuterium nuclear magnetic resonance (2H NMR) spectra of specifically head-group- and chain-deuterated ester- and ether-linked phosphatidylcholine bilayers were studied as a function of temperature over the range -33 to 50 degrees C. Head-group-deuterated dihexadecylphosphatidylcholine ([alpha-2H2]DHPC) bilayers yield line shapes and spin-lattice relaxation times similar to those observed for its ester-linked counterpart, dipalmitoylphosphatidylcholine ([alpha-2H2]DPPC), in the high-temperature… Expand
Phospholipids chiral at phosphorus. Dramatic effects of phosphorus chirality on the deuterium NMR properties of the choline head group of phospholipids in the liquid crystalline phase.
TLDR
The results suggest that the Rp isomer is more rigid than the other isomers in the choline head group and there are dramatic differences between the spectra of DPPC and different isomers of DPPsC in the liquid crystalline phase. Expand
The effect of cholesterol on lipid dynamics and packing in diether phosphatidylcholine bilayers. X-ray diffraction and 2H-NMR study.
TLDR
The low-angle lamellar reflections of DHPC at 22 degrees C in the absence of cholesterol are indicative of an interdigitated phase, demonstrating that cholesterol facilitates the conversion from aninterdigitated to a non-interdigitate phase. Expand
Thermotropic and barotropic phase transitions in bilayer membranes of ether-linked phospholipids with varying alkyl chain lengths.
TLDR
It was proved that the phase behavior of the ester-linked PC bilayers under high temperature and pressure is almost equivalent to that of the ether-linkedPC bilayers in the vicinity of ambient pressure. Expand
Molecular order and dynamics of diphytanylglycerol phospholipids: a 2H and 31P-NMR study☆
2H NMR and 31P-NMR have been applied to study the conformational and organizational properties of synthetically deuterated dihexadecylglycerol phosphate and diphytanylglycerol phosphate, and ofExpand
High-pressure infrared spectroscopy of ether- and ester-linked phosphatidylcholine aqueous dispersions.
TLDR
At all pressures where the correlation field component band delta'CH2 can be resolved, the relative peak height/intensity ratio R = I delta'/I delta is greater in DPPC than in DHPC, suggesting that this parameter may be useful as a test of interdigitation. Expand
Time-resolved emission spectra and anisotropy profiles for symmetric diacyl- and dietherphosphatidylcholines
The solvent relaxation behavior of Patman (6-palmitoyl-2-[[2-(trimethylammonium) ethyl]methylamino]naphthalene chloride) was investigated in small unilamellar vesicles composed of symmetric diacyl(Expand
Structure and thermotropic properties of hydrated 1-eicosyl-2-dodecyl-rac-glycero-3-phosphocholine and 1-dodecyl-2-eicosyl-rac-glycero-3-phosphocholine bilayer membranes.
TLDR
The ability of both EDPC and DEPC to form triple-chain interdigitated gel-state bilayers suggests that the conformational inequivalence at the sn-1 and sn-2 positions is less pronounced in the ether-linked PCs compared to the ester- linked PCs, where only one of the positional isomers isomers. Expand
Bilayer interactions of ether- and ester-linked phospholipids: dihexadecyl- and dipalmitoylphosphatidylcholines.
TLDR
A composition-dependent gel----gel transition between interdigitated and noninterdigitated bilayers is observed at T less than TP, and this leads to eutectic behavior of the DHPC/DPPC system. Expand
A new monofluorinated phosphatidylcholine forms interdigitated bilayers.
TLDR
F-DPPC is the first phosphoglyceride containing sn-1 and sn-2 ester-linked fatty acyl chains of equal length that spontaneously forms interdigitated bilayers in the gel state in the absence of inducing agents such as alcohols. Expand
Differential scanning calorimetry studies of phosphonolipid analogs of lung surfactant glycerophospholipids
Abstract A series of synthetic analogs of naturally occurring glycerophospholipids has been studied for gel-to-liquid transition temperature (Tc) by using differential scanning calorimetry. TheExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 43 REFERENCES
The origin of multiple quadrupole couplings in the deuterium NMR spectra of the 2 chain of 1,2 dipalmitoyl‐sn‐glycero‐3‐phosphorylcholine
TLDR
Deuterium NMR spectra of lipid dideuterated at the C-2 position in the ~2-2 chain generally contain two quadrupole coupling constants, which are observed in bilayer dispersions of DPPC and of several other 1,2-acyl phospholipids and also when lipids are incorporated into membranes in bac- teria. Expand
Comparative study of the gel phases of ether- and ester-linked phosphatidylcholines.
TLDR
X-ray diffraction studies indicate that DHPC dispersions form a lamellar gel phase with dav congruent to 46 A both above and below the "subtransition", and it is suggested that the reduced dav observed for DHPC is due to an interdigitated lamellary gel phase which exists at all temperatures below the pretransition at 35 degrees C. Expand
Molecular dynamics and conformation in the gel and liquid-crystalline phases of phosphatidylethanolamine bilayers.
TLDR
Solid-state deuterium and carbon-13 nuclear magnetic resonance (NMR) spectra used to study the molecular dynamics and conformation of dipalmitoylphosphatidylethanolamine in both the gel and liquid-crystalline phases suggest that the backbone is in a gauche conformation in the gel state, rather than a trans conformation such as found in single crystals. Expand
Neutron diffraction studies on selectively deuterated phospholipid bilayers
TLDR
The mean position of the deuterated segments within the membrane can be determined in most cases to a precision of better than ±1 Å, and the average orientation of the phosphocholine group in the gel state as well as the liquid crystalline state is almost parallel to the membrane surface. Expand
Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance studies of N-palmitoylglucosylceramide (cerebroside) head group structure.
TLDR
The results indicate a rather mobile structure for the cerebroside head group, in a variety of environments, and where multiple solutions do occur, they fall within a narrow orientational subspace so that all solutions exhibit the same general features. Expand
Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance investigation of deuterium-labelled N-hexadeconoylgalactosylceramides (cerebrosides).
TLDR
The results suggest the presence of a hydrogen bond network in the polar headgroup region of palmitoylgalactosylceramide, leading to the possibility of characterising the cerebroside-cholesterol phase diagram using deuterium nuclear magnetic resonance spectroscopy. Expand
The polar group conformation of 1,2-dialkyl phosphatidylcholines An NMR study
Abstract The polar group conformation of 1,2- di -O- alkylglycerophosphocholine (dialkylphosphatidylcholine (ether phosphatidylcholine)) is investigated and compared to that of the correspondingExpand
Conformation and motion of the choline head group in bilayers of dipalmitoyl-3-sn-phosphatidylcholine.
The conformation and motion of the choline head group in lipid bilayers above and below the gel-to-liquid crystal transition point are studied by means of deuterium and phosphorus magnetic resonance.Expand
Bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Conformational differences between the fatty acyl chains.
TLDR
It is concluded that the beginnings of the two chains have orientations parallel and perpendicular to the bilayer normal, suggesting the possibility of two long-lived conformations of the glycerol constituent. Expand
Neutron diffraction studies on phosphatidylcholine model membranes. II. Chain conformation and segmental disorder.
Abstract In this paper neutron diffraction experiments on 1,2-dipalmitoyl- sn -glycero-3-phosphocholine selectively deuterated in the hydrocarbon chains are reported. The experiments were carried outExpand
...
1
2
3
4
5
...